【Animal Modeling】-Constructing severe combined immunodeficiency mice based on CRISPR/Cas9 technology

To use CRISPR/Cas9 technology to target knockout Rag2 gene encoding mouse T and B cells and IL2rg gene encoding NK cells, and construct T, B cells and NK cells combined immunodeficiency mice...

2022-03-16

【Animal modeling】-GGTA1-/- Wuzhishan miniature pig SLA class I gene molecular characteristics and similarity analysis with HLA

To clarify the molecular structure of SLA class I gene of Wuzhishan miniature pig with α-1,3 galactosyltransferase gene knockout (GGTA1-/-) and its similarity with human HLA, and to study the cellularity of xenotransplantation. Rejection is important...

2022-03-16

【Animal modeling】-Changes of biological characteristics of black-lined hamsters infected with Babesia

To establish a black-lined hamster model infected with babesiosis, to clarify the changes of biological characteristics of black-lined hamsters after infection, and to provide basic data for the detection and prevention of babesiosis...

2022-03-16

【Animal modeling】-PCR detection of Helicobacter pylori and \"Helicobacter macaque\" infection in experimental rhesus monkeys and cynomolgus monkeys

To investigate the infection of Helicobacter pylori and \"Helicobacter rhesus monkeys\" in domestic experimental rhesus monkeys and cynomolgus monkeys...

2022-03-15

【Animal modeling】-Depolarization rebound and regulatory mechanism of glial neurons in the dorsal horn of rat spinal cord

To study the depolarization rebound and its regulatory mechanism of the colloidal (SG) neurons in the dorsal horn of the spinal cord in rats, in order to provide a reference for the clinical treatment of depolarization rebound related diseases...

2022-03-15

【Animal modeling】-Analysis of clinical characteristics of mice infected with parvovirus

To analyze the distribution law, detoxification and antibody changes of mouse parvovirus in artificially infected mice, and the situation of MVM infection in mice under natural conditions...

2022-03-15

【Animal modeling】-Prokaryotic expression of monkey D-type retrovirus p27 gene and establishment of flow immunofluorescence microsphere detection technology

To construct the p27 protein of simian D-type retrovirus, and establish a flow immunofluorescence microsphere detection technology for the detection of simian D-type retrovirus...

2022-03-14

【Animal Modeling】-Establishment of a fluorescent PCR detection method for mouse polyoma virus and its application in the investigation of infection in naked mole rats

To establish a quantitative PCR detection method for polyoma virus, and to investigate the infection of polyoma virus in naked mole rats...

2022-03-14

【Animal Modeling】-Analysis of Transcriptional Levels of CMAH Gene in Different Tissues of Mice

The sialic acid and its derivatives contained in mammalian cells mainly include N-acetylneuraminic acid, N-glycolylneuraminic acid and deaminated neuraminic acid. The synthesis of Neu5Gc under the catalysis of acid hydroxylase? In this study, the rel...

2022-03-14

【Animal modeling】-Analysis of genetic characteristics of BABL/c mutant curly hair mice by microsatellite DNA markers

To analyze the genetic differences between BALB/c mutant curly-haired mice and normal BALB/c mice using microsatellite DNA marker technology...

2022-03-14

【Animal Modeling】-Establishment of a Fluorescent Quantitative Detection Method for Suspected Taylor\'s Virus in Rats

To establish a rapid and accurate method for the clinical detection of suspected Theiler\'s-like virus of rats (TLV) in rats, using TaqMan probe fluorescence quantitative polymerase chain reaction (qPCR) technology, specific Sexual detection of TLV v...

2022-03-12

【Animal modeling】-Establishment and application of multiplex PCR method for four pathogens in experimental animals

To establish a multiplex PCR method for the rapid detection of four experimental animal pathogens, Pasteurella multocida, Bordetella bronchiscus, Mycoplasma and Klebsiella pneumoniae...

2022-03-12

【Animal Modeling】-Determination and Analysis of Hematology and Serum Biochemical Indexes in African Green Monkeys

To detect the hematology and serum biochemical indexes of 50 healthy African green monkeys, and to analyze the hematology and serum biochemistry of African green monkeys of different genders and different age groups (young and juvenile group: 1-3 yea...

2022-03-12

【Animal modeling】-Establishment and application of RT-PCR method for bovine viral diarrhea virus

To establish a double RT-PCR method for the detection of bovine viral diarrhea virus (BVDV) in bovine samples...

2022-03-12

【Animal modeling】-Establishment and application of Salmonella LAMP detection method in tree shrew feces

To establish a loop-mediated isothermal amplification detection method for Salmonella in tree shrew feces, and to make a preliminary application of the method...

2022-03-12

【Animal Modeling】-CBA/CaJ Mice Organ Weight, Blood Physiological and Biochemical Indexes and Immune Cell Proportion

To determine the basic biological indicators of SPF CBA/CaJ mice, and to lay a foundation for better use of this strain of mice for biomedical research...

2022-03-10