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How to anesthetize small pigs?

How to anesthetize small pigs?

Fasting before anesthesia. Miniature pigs should be observed for 5-7 days before anesthesia. Dehydration and weight loss may be caused during transportation. Standard care procedures should be established for newly introduced miniature pigs....

2020-10-15
How to prepare a small pig deaf animal model?

How to prepare a small pig deaf animal model?

The species difference between minipigs and humans is very small. They are relatively close to humans in terms of organ structure and metabolism. Many diseases share common causes with humans. It is an ideal animal model for studying various human di...

2020-10-15
How to cultivate minipig parotid gland cells?

How to cultivate minipig parotid gland cells?

4-month-old experimental mini-pigs were subjected to conventional anesthesia. Under aseptic conditions, the parotid alveolar tissue was extracted, the glandular lobule envelope and other binding tissues were removed, and then immersed in HamF12 mediu...

2020-10-15
How to grow periodontal ligament cells of miniature pigs?

How to grow periodontal ligament cells of miniature pigs?

A 6-month-old miniature pig weighs about 25 kg. Miniature pigs will be anesthetized regularly. Pull out the eight anterior teeth (4 dogs and 4 incisions) of the upper and lower jaws of the miniature pig under aseptic conditions, and immediately put t...

2020-10-15
How to cultivate miniature pig dental pulp stem cells?

How to cultivate miniature pig dental pulp stem cells?

How to cultivate miniature pig dental pulp stem cells?...

2020-10-15
What are the steps to extract plasmid DNA by alkaline lysis?

What are the steps to extract plasmid DNA by alkaline lysis?

What are the steps to extract plasmid DNA by alkaline lysis?...

2020-10-14
How to configure the nutrient solution for experiment?

How to configure the nutrient solution for experiment?

How to configure the nutrient solution for experiment?...

2020-10-14
How to do ether anesthesia experiment in rats and mice?

How to do ether anesthesia experiment in rats and mice?

How to do ether anesthesia experiment in rats and mice?...

2020-10-14
What is the method of genotyping?

What is the method of genotyping?

Genotype (genetic type) refers to the type of genes related to the traits contained in the DNA of an organism...

2020-10-14
What is embryonic stem cell method?

What is embryonic stem cell method?

The Martin Evans Institute has successfully cultivated a pluripotent stem cell line from the internal cell mass of early embryos, which can grow in vitro and can differentiate into other cells even if it is reinjected into germ cysts , These cells (i...

2020-10-14
What is the retroviral vector infection method?

What is the retroviral vector infection method?

Studies have shown that retroviruses, especially lentiviral vectors, can effectively introduce foreign DNA into egg cells...

2020-10-14
What is DNA microinjection?

What is DNA microinjection?

DNA microinjection is the earliest, most mature and most widely used transgenic technology...

2020-10-14
What is a point-specific recombination system?

What is a point-specific recombination system?

\"Point-specific recombination system\" includes Cre-LoxP system and FLP-FRT system. So far, the most widely used method is Cre/LoxP-mediated point-specific recombination system...

2020-10-14
What is an inducible gene expression system?

What is an inducible gene expression system?

Inducible Gene Expression System (Inducible Gene Expression System) allows introduced genes to regulate gene expression levels at a specific time or on specific tissues or cell types...

2020-10-14
How to prepare an animal model of transplanted tumor?

How to prepare an animal model of transplanted tumor?

The blood samples of clinical leukemia patients cultured and passaged in the abdominal cavity of mice, bone marrow samples or non-solid tumor ascites samples are equal amounts of balanced salt solution (PBS, etc.)...

2020-10-14
How to extract DNA correctly?

How to extract DNA correctly?

How to extract DNA correctly?...

2020-10-14