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How was the animal model of ulcerative colitis induced by dinitrochlorobenzene prepared?

How was the animal model of ulcerative colitis induced by dinitrochlorobenzene prepared?

Replication method In adult male BALB/c mice, cut abdominal hair (without damage to the skin) into approximately 2.0 cm x 1.5 cm and 3.3% 2,4-dinitrochlorobenzene (DNCB) acetone olive oil. Solution (1:1 ratio) abdominal smear, once a day, 50μl a day,...

2020-08-07
How to prepare an animal model of Crohn disease?

How to prepare an animal model of Crohn disease?

Replication method: Adult rats fasted for 48 hours were anesthetized by intraperitoneal injection of sodium pentobarbital, a newborn gastric tube was inserted to a depth of about 8 cm, and trinitrobenzene sulfonic acid (2, 4,6-trinitrobenzene) Benzen...

2020-08-07
How to prepare an animal model of appendicitis?

How to prepare an animal model of appendicitis?

Reproduction method After anesthesia, the adult rat is fixed, the abdomen is opened under aseptic conditions, the intestine connected with the appendix, terminal ileum and appendix mesentery (hereinafter referred to as the adjacent intestine) is take...

2020-08-07
How to prepare an animal model of stress duodenal ulcer?

How to prepare an animal model of stress duodenal ulcer?

Breeding method Adult rats fasted in water for 16 hours were anesthetized by intraperitoneal injection of sodium pentobarbital, fixed, and then covered with electric clippers to cover a slightly wider area than the burned site...

2020-08-07
How to prepare animal model of chronic gastric ulcer induced by acetic acid cautery method?

How to prepare animal model of chronic gastric ulcer induced by acetic acid cautery method?

Reproduction method Anesthetize adult rats with sodium pentobarbital, incise the abdomen in the supine position, and inject 0.01 to 0.05ml of 20% to 100% acetic acid into the anterior wall of the gastric antrum with a 0.01ml tuberculin syringe...

2020-08-07
How to prepare an animal model of acute gastric ulcer induced by pyloric ligation?

How to prepare an animal model of acute gastric ulcer induced by pyloric ligation?

Replication method Adult rats, fasted and fed water for 48 hours, anesthetized with ether, incised the abdominal wall to expose the stomach, pull the stomach out of the abdominal cavity with blunt forceps, and suture the junction of the pylorus and d...

2020-08-07
How to prepare an animal model of acute gastric ulcer induced by chemical substances?

How to prepare an animal model of acute gastric ulcer induced by chemical substances?

Reproduction method: adult experimental mice, fasting for 48 hours, oral gavage of ethanol (5 ml/kg body weight) or acetylsalicylic acid (200 mg/kg body weight) or 0.6 mol/L hydrochloric acid (10 ml/kg body weight) 1 After hours, kill the animal by c...

2020-08-07
How to prepare an animal model of chronic atrophic gastritis induced by immune method?

How to prepare an animal model of chronic atrophic gastritis induced by immune method?

Replication method Adult rats, subcutaneously injected 0.3ml/emulsion, made from the same strain of rat gastric mucosa, which contains the same saline tissue homogenate and 1:1 adjuvant, injected subcutaneously, repeated every 3-4 weeks...

2020-08-07
How to prepare an animal model of chronic atrophic gastritis induced by chemical substances?

How to prepare an animal model of chronic atrophic gastritis induced by chemical substances?

Reproductive method Put 0.05% methylnitrosonitrosoguanidine (MNNG) into a drinking bottle for a 5-6 week old rat, and drink 50 ml per mouse per day for 1 month. Or pour 0.01 to 0.02% ammonia water into a water bottle and drink it for 3 to 6 months fo...

2020-08-07
How to prepare an animal model of chronic atrophic gastritis induced by physical factors?

How to prepare an animal model of chronic atrophic gastritis induced by physical factors?

Reproduction method Adult rats take 10 ml/kg body weight once a day in 15% sodium chloride (NaCl) solution at 55°C or simple distilled water at 55°C for 12 to 32 weeks...

2020-08-07
How to prepare an animal model of acute gastritis?

How to prepare an animal model of acute gastritis?

Replication method Adult male rats, fasted for 48 hours, no water, acetylsalicylic acid 100 mg/kg body weight or 10 mmol/L acetic acid or different concentrations of hydrochloric acid (1, 10, 10 mmol/L) or the same type of bile Or 2 mmol. /L Taurine ...

2020-08-07
How to prepare an animal model for cardiopulmonary bypass?

How to prepare an animal model for cardiopulmonary bypass?

Replication method Rats were intraperitoneally injected with sodium pentobarbital at a dose of 30 mg/kg body weight, intubated through the mouth and mechanically ventilated (frequency 60 times)...

2020-08-06
How to prepare an animal model of coronary artery spasm caused by ovariectomy?

How to prepare an animal model of coronary artery spasm caused by ovariectomy?

Reproduction method The female rhesus monkey is surgically removed from the female ovary, the coronary artery (hereinafter referred to as the coronary artery) is contracted using a vasoconstrictor, and the difference with humans is compared by corona...

2020-08-06
How to prepare animal model of right atrial hypertrophy caused by thoracotomy?

How to prepare animal model of right atrial hypertrophy caused by thoracotomy?

Feeding method Under anesthesia, a dog weighing 20-30 kg is injected intravenously with 30 mg/kg sodium pentobarbital, fixed in the right supine position, and intubated through positive pressure ventilation...

2020-08-06
[Animal model]-Rat model of vasculitis obliterans induced by immune method

[Animal model]-Rat model of vasculitis obliterans induced by immune method

Thromboangiitis obliterans is a peripheral vascular disease dominated by autoimmunity, smoking, cold and other external factors and sex hormones are the main cause of the disease. The following describes the method of using the above-mentioned pathog...

2020-08-06
How to prepare an animal model of lauric acid-induced vasculitis obliterans in rats?

How to prepare an animal model of lauric acid-induced vasculitis obliterans in rats?

Reproduction method Anesthetize male Wistar rats weighing 280-320g by intraperitoneal injection of 35 mg/kg body weight of 3% pentobarbital sodium...

2020-08-06